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Enzymatic Characterization of a Mutant of Escherichia coli with an Altered DNA Ligase

A temperature-sensitive, radiation-sensitive mutant of Escherichia coli has been assayed for DNA ligase activity in vitro. The strain contains a markedly reduced amount of DNA-joining activity, which is thermolabile. The formation of the ligase-adenylate intermediate is also temperature-sensitive in...

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Main Authors: Modrich, Paul, Lehman, I. R.
格式: Artigo
語言:Inglês
出版: 1971
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC389100/
https://ncbi.nlm.nih.gov/pubmed/4995816
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