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Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II
The major DNA-synthesizing enzyme present in Pol A(1)(-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4)(+), and native DNA for maximal activity. The enzyme...
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| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1971
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC389037/ https://ncbi.nlm.nih.gov/pubmed/4927672 |
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