Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II
The major DNA-synthesizing enzyme present in Pol A(1)(-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4)(+), and native DNA for maximal activity. The enzyme...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1971
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389037/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4927672/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.68.4.761 |
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