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Development and Accuracy of Quantitative Real-Time Polymerase Chain Reaction Assays for Detection and Quantification of Enterotoxigenic Escherichia coli (ETEC) Heat Labile and Heat Stable Toxin Genes in Travelers' Diarrhea Samples

Enterotoxigenic Escherichia coli (ETEC), the leading bacterial pathogen of travelers' diarrhea, is routinely detected by an established DNA hybridization protocol that is neither sensitive nor quantitative. Quantitative real-time polymerase chain reaction (qPCR) assays that detect the ETEC toxi...

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Detalhes bibliográficos
Main Authors: Youmans, Bonnie P., Ajami, Nadim J., Jiang, Zhi-Dong, Petrosino, Joseph F., DuPont, Herbert L., Highlander, Sarah K.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society of Tropical Medicine and Hygiene 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3886408/
https://ncbi.nlm.nih.gov/pubmed/24189361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4269/ajtmh.13-0383
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