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Probing Enzymatic Activity Inside Single Cells
We report a novel approach for determining the enzymatic activity within a single suspended cell. Using a steady-state microfluidic delivery device, and timed exposure to the pore-forming agent digitonin, we controlled the plasma membrane permeation of individual NG108-15 cells. Mildly permeabilized...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3882690/ https://ncbi.nlm.nih.gov/pubmed/24003961 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac4013122 |
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