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Probing Enzymatic Activity Inside Single Cells

We report a novel approach for determining the enzymatic activity within a single suspended cell. Using a steady-state microfluidic delivery device, and timed exposure to the pore-forming agent digitonin, we controlled the plasma membrane permeation of individual NG108-15 cells. Mildly permeabilized...

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Sonraí Bibleagrafaíochta
Main Authors: Olofsson, Jessica, Xu, Shijun, Jeffries, Gavin D. M., Jesorka, Aldo, Bridle, Helen, Isaksson, Ida, Weber, Stephen G, Orwar, Owe
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2013
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3882690/
https://ncbi.nlm.nih.gov/pubmed/24003961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac4013122
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