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A strategy of exon shuffling for making large peptide repertoires displayed on filamentous bacteriophage.

It has been suggested that recombination and shuffling between exons has been a key feature in the evolution of proteins. We propose that this strategy could also be used for the artificial evolution of proteins in bacteria. As a first step, we illustrate the use of a self-splicing group I intron wi...

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Autors principals: Fisch, I, Kontermann, R E, Finnern, R, Hartley, O, Soler-Gonzalez, A S, Griffiths, A D, Winter, G
Format: Artigo
Idioma:Inglês
Publicat: 1996
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC38821/
https://ncbi.nlm.nih.gov/pubmed/8755549
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