Conditions for Using DNA Polymerase I as an RNA-Dependent DNA Polymerase
Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The molar ratio of enzyme to template is critical; below a certain level, synthesis is not observed. Hybri...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1974
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388156/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4133845/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.4.1035 |
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