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Conditions for Using DNA Polymerase I as an RNA-Dependent DNA Polymerase

Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The molar ratio of enzyme to template is critical; below a certain level, synthesis is not observed. Hybri...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Gulati, S. C., Kacian, D. L., Spiegelman, S.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1974
Fag:
Biological Sciences: Biochemistry
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388156/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4133845/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.4.1035
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