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Conditions for Using DNA Polymerase I as an RNA-Dependent DNA Polymerase

Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The molar ratio of enzyme to template is critical; below a certain level, synthesis is not observed. Hybri...

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Autors principals: Gulati, S. C., Kacian, D. L., Spiegelman, S.
Format: Artigo
Idioma:Inglês
Publicat: 1974
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC388156/
https://ncbi.nlm.nih.gov/pubmed/4133845
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