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Fluorotyrosine Alkaline Phosphatase from Escherichia coli: Preparation, Properties, and Fluorine-19 Nuclear Magnetic Resonance Spectrum

Alkaline phosphatase (EC 3.1.3.1) containing m-flurotyrosine has been prepared from E. coli grown in the presence of m-flurotyrosine. The kinetic properties of the m-fluorotyrosine enzyme measured with p-nitrophenylphosphate at pH 8.0 and dinitrophenylphosphate at pH 5.5 are essentially the same as...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Principais autores: Sykes, Brian D., Weingarten, Harold I., Schlesinger, Milton J.
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1974
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388028/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4592693/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.2.469
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