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Fluorotyrosine Alkaline Phosphatase from Escherichia coli: Preparation, Properties, and Fluorine-19 Nuclear Magnetic Resonance Spectrum

Alkaline phosphatase (EC 3.1.3.1) containing m-flurotyrosine has been prepared from E. coli grown in the presence of m-flurotyrosine. The kinetic properties of the m-fluorotyrosine enzyme measured with p-nitrophenylphosphate at pH 8.0 and dinitrophenylphosphate at pH 5.5 are essentially the same as...

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Detalhes bibliográficos
Main Authors: Sykes, Brian D., Weingarten, Harold I., Schlesinger, Milton J.
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC388028/
https://ncbi.nlm.nih.gov/pubmed/4592693
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