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Localization of Polyribosomes Containing Alkaline Phosphatase Nascent Polypeptides on Membranes of Escherichia coli

A procedure has been developed for extracting membranes from bacterial cells under conditions that keep a large fraction of bacterial polyribosomes intact. Freeze-thawing spheroplasts in the presence of deoxyribonuclease, followed by differential centrifugation, permits a separation of free and memb...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Main Authors: Cancedda, Ranieri, Schlesinger, Milton J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1974
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC246556/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4587609/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.117.1.290-301.1974
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