Localization of Polyribosomes Containing Alkaline Phosphatase Nascent Polypeptides on Membranes of Escherichia coli

A procedure has been developed for extracting membranes from bacterial cells under conditions that keep a large fraction of bacterial polyribosomes intact. Freeze-thawing spheroplasts in the presence of deoxyribonuclease, followed by differential centrifugation, permits a separation of free and memb...

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Εκδόθηκε σε:	J Bacteriol
Κύριοι συγγραφείς:	Cancedda, Ranieri, Schlesinger, Milton J.
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	American Society for Microbiology (ASM) 1974
Θέματα:	Physiology and Metabolism
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC246556/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4587609/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.117.1.290-301.1974
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