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Native cysteine residues are dispensable for the structure and function of all five yeast mitotic septins

Budding yeast septins assemble into hetero-octamers and filaments required for cytokinesis. Solvent-exposed cysteine (Cys) residues provide sites for attaching substituents useful in assessing assembly kinetics and protein interactions. To introduce Cys at defined locations, site-directed mutagenesi...

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Detalhes bibliográficos
Main Authors: de Val, Natalia, McMurray, Michael A., Lam, Lisa H., Hsiung, Chris C.-S., Bertin, Aurélie, Nogales, Eva, Thorner, Jeremy
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3880206/
https://ncbi.nlm.nih.gov/pubmed/23775754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prot.24345
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