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Biarsenical ligands bind to endogenous G-protein α-subunits and enable allosteric sensing of nucleotide binding
BACKGROUND: Heterotrimeric G-proteins relay extracellular signals to intracellular effector proteins. Multiple methods have been developed to monitor their activity; including labeled nucleotides and biosensors based on genetically engineered G-proteins. Here we describe a method for monitoring unla...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
BioMed Central
2013
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3878488/ https://ncbi.nlm.nih.gov/pubmed/24344803 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-14-37 |
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