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Biarsenical ligands bind to endogenous G-protein α-subunits and enable allosteric sensing of nucleotide binding

BACKGROUND: Heterotrimeric G-proteins relay extracellular signals to intracellular effector proteins. Multiple methods have been developed to monitor their activity; including labeled nucleotides and biosensors based on genetically engineered G-proteins. Here we describe a method for monitoring unla...

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Detalhes bibliográficos
Main Authors: Tõntson, Lauri, Kopanchuk, Sergei, Rinken, Ago
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3878488/
https://ncbi.nlm.nih.gov/pubmed/24344803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-14-37
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