Base Preferences in Non-Templated Nucleotide Incorporation by MMLV-Derived Reverse Transcriptases

Reverse transcriptases derived from Moloney Murine Leukemia Virus (MMLV) have an intrinsic terminal transferase activity, which causes the addition of a few non-templated nucleotides at the 3´ end of cDNA, with a preference for cytosine. This mechanism can be exploited to make the reverse transcript...

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Main Authors: Zajac, Pawel, Islam, Saiful, Hochgerner, Hannah, Lönnerberg, Peter, Linnarsson, Sten
格式: Artigo
語言:Inglês
出版: Public Library of Science 2013
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3877366/
https://ncbi.nlm.nih.gov/pubmed/24392002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0085270
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