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Detection of the Escherichia coli Group 2 Polysaccharide Capsule Synthesis Gene kpsM by a Rapid and Specific PCR-Based Assay

A rapid and simple PCR-based assay for detection of the group 2 capsule synthesis gene kpsM of Escherichia coli was designed and validated. When combined with the published group 2 primers (kpsIIf, 5′-GCGCATTTGCTGATACTGTTG-3′; kpsIIr, 5′-CATCCAGACGATAAGCATGAGCA-3′), the new primers (the kpsIIf prime...

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Detalhes bibliográficos
Main Authors: Johnson, James R., O'Bryan, Timothy T.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC387594/
https://ncbi.nlm.nih.gov/pubmed/15071046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.4.1773-1776.2004
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