De novo transcript sequence reconstruction from RNA-Seq: reference generation and analysis with Trinity

De novo assembly of RNA-Seq data allows us to study transcriptomes without the need for a genome sequence, such as in non-model organisms of ecological and evolutionary importance, cancer samples, or the microbiome. In this protocol, we describe the use of the Trinity platform for de novo transcript...

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Detalhes bibliográficos
Main Authors: Haas, Brian J., Papanicolaou, Alexie, Yassour, Moran, Grabherr, Manfred, Blood, Philip D., Bowden, Joshua, Couger, Matthew Brian, Eccles, David, Li, Bo, Lieber, Matthias, MacManes, Matthew D., Ott, Michael, Orvis, Joshua, Pochet, Nathalie, Strozzi, Francesco, Weeks, Nathan, Westerman, Rick, William, Thomas, Dewey, Colin N., Henschel, Robert, LeDuc, Richard D., Friedman, Nir, Regev, Aviv
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3875132/
https://ncbi.nlm.nih.gov/pubmed/23845962
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.084
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