A Novel Methylation PCR that Offers Standardized Determination of FMR1 Methylation and CGG Repeat Length without Southern Blot Analysis

Fragile X syndrome and associated disorders are characterized by the number of CGG repeats and methylation status of the FMR1 gene for which Southern blot (SB) historically has been required for analysis. This study describes a simple PCR-only workflow (mPCR) to replace SB analysis, that incorporate...

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Bibliographic Details
Main Authors: Grasso, Marina, Boon, Elles M.J., Filipovic-Sadic, Stela, van Bunderen, Patrick A., Gennaro, Elena, Cao, Ru, Latham, Gary J., Hadd, Andrew G., Coviello, Domenico A.
Format: Artigo
Language:Inglês
Published: American Society for Investigative Pathology 2014
Subjects:
Technical Advance
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3873488/
https://ncbi.nlm.nih.gov/pubmed/24177047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2013.09.004
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