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Reconstitution of the 26S proteasome reveals functional asymmetries in its AAA+ unfoldase

The 26S proteasome is the major eukaryotic ATP-dependent protease, yet the detailed mechanisms utilized by the proteasomal heterohexameric AAA+ unfoldase to drive substrate degradation remain poorly understood. To perform systematic mutational analyses of individual ATPase subunits, we heterologousl...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Beckwith, Robyn, Estrin, Eric, Worden, Evan J., Martin, Andreas
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3869383/
https://ncbi.nlm.nih.gov/pubmed/24013205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.2659
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