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Cloning and expression of Bradyrhizobium japonicum uptake hydrogenase structural genes in Escherichia coli.

To identify the structural genes for the components of Bradyrhizobium japonicum uptake hydrogenase (Mr 60,000 and 30,000), we have expressed these genes in Escherichia coli and shown that the products cross-react with antibodies to the respective hydrogenase subunits. We constructed subclones of ove...

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Bibliographic Details
Main Authors: Zuber, M, Harker, A R, Sultana, M A, Evans, H J
Format: Artigo
Language:Inglês
Published: 1986
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC386782/
https://ncbi.nlm.nih.gov/pubmed/3532119
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