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Cloning and expression of Bradyrhizobium japonicum uptake hydrogenase structural genes in Escherichia coli.

To identify the structural genes for the components of Bradyrhizobium japonicum uptake hydrogenase (Mr 60,000 and 30,000), we have expressed these genes in Escherichia coli and shown that the products cross-react with antibodies to the respective hydrogenase subunits. We constructed subclones of ove...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Zuber, M, Harker, A R, Sultana, M A, Evans, H J
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386782/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3532119/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.20.7668
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