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Insert sequence length determines transfection efficiency and gene expression levels in bicistronic mammalian expression vectors
Bicistronic expression vectors have been widely used for co-expression studies since the initial discovery of the internal ribosome entry site (IRES) about 25 years ago. IRES sequences allow the 5’ cap-independent initiation of translation of multiple genes on a single messenger RNA strand. Using a...
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Autores principales: | , , , |
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Formato: | Artigo |
Lenguaje: | Inglês |
Publicado: |
e-Century Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3867706/ https://ncbi.nlm.nih.gov/pubmed/24380024 |
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