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Insert sequence length determines transfection efficiency and gene expression levels in bicistronic mammalian expression vectors

Bicistronic expression vectors have been widely used for co-expression studies since the initial discovery of the internal ribosome entry site (IRES) about 25 years ago. IRES sequences allow the 5’ cap-independent initiation of translation of multiple genes on a single messenger RNA strand. Using a...

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Bibliografiske detaljer
Main Authors: Payne, Andrew J, Gerdes, Bryan C, Kaja, Simon, Koulen, Peter
Format: Artigo
Sprog:Inglês
Udgivet: e-Century Publishing Corporation 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3867706/
https://ncbi.nlm.nih.gov/pubmed/24380024
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