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Fluorescence Imaging-Based High-Throughput Screening of Fast- and Slow-Cycling LOV Proteins
Light-oxygen-voltage (LOV) domains function as blue light-inducible molecular switches. The photosensory LOV domains derived from plants and fungi have provided an indispensable tool for optogenetics. Here we develop a high-throughput screening system to efficiently improve switch-off kinetics of LO...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Public Library of Science
2013
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3867380/ https://ncbi.nlm.nih.gov/pubmed/24367542 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0082693 |
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