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The Effect of Antigen Retrieval on Cells Fixed in 10% Neutral Buffered Formalin Followed by Transfer to 70% Ethanol

Fixation in 10% neutral buffered formalin prior to transfer to 70% ethanol for one week has been shown to adequately preserve immunorecognition of PCNA, cytokeratins AE1/AE3 and EGFr. This study investigated whether 12 hrs fixation in 10% NBF plus transfer to 70% ethanol for 4 weeks would similarly...

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Autors principals: Otali, Dennis, He, Qinghua, Grizzle, William E.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3866107/
https://ncbi.nlm.nih.gov/pubmed/24358179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0082405
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