Both the 25-kDa and 50-kDa domains in myosin subfragment 1 are close to the reactive thiols.
The thiol-specific photoactivatable reagent benzophenone-4-iodoacetamide can be incorporated into myosin subfragment 1 (S1), accompanied by an increase of Ca2+-ATPase and the loss of K+-ATPase activities, a characteristic property of S1 when reactive sulfhydryl 1 (SH-1) is modified. After trypsin cl...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1986
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386509/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2944108/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.17.6392 |
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