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Counting unstained, confluent cells by modified bright-field microscopy

We present a very simple procedure yielding high-contrast images of adherent, confluent cells such as human neuroblastoma (SH-EP) cells by ordinary bright-field microscopy. Cells are illuminated through a color filter and a pinhole aperture placed between the condenser and the cell culture surface....

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Autors principals: Drey, L. Louis, Graber, Michael C., Bieschke, Jan
Format: Artigo
Idioma:Inglês
Publicat: 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3864689/
https://ncbi.nlm.nih.gov/pubmed/23834382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114056
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