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Counting unstained, confluent cells by modified bright-field microscopy
We present a very simple procedure yielding high-contrast images of adherent, confluent cells such as human neuroblastoma (SH-EP) cells by ordinary bright-field microscopy. Cells are illuminated through a color filter and a pinhole aperture placed between the condenser and the cell culture surface....
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| Main Authors: | , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2013
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3864689/ https://ncbi.nlm.nih.gov/pubmed/23834382 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114056 |
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