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Mutant HbpR transcription activator isolation for 2‐chlorobiphenyl via green fluorescent protein‐based flow cytometry and cell sorting

Mutants were produced in the A‐domain of HbpR, a protein belonging to the XylR family of σ(54)‐dependent transcription activators, with the purpose of changing its effector recognition specificity from 2‐hydroxybiphenyl (2‐HBP, the cognate effector) to 2‐chlorobiphenyl (2‐CBP). Mutations were introd...

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Hlavní autoři: Beggah, Siham, Vogne, Christelle, Zenaro, Elena, Van Der Meer, Jan Roelof
Médium: Artigo
Jazyk:Inglês
Vydáno: Blackwell Publishing Ltd 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3864433/
https://ncbi.nlm.nih.gov/pubmed/21261823
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2007.00008.x
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