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Mutant HbpR transcription activator isolation for 2‐chlorobiphenyl via green fluorescent protein‐based flow cytometry and cell sorting
Mutants were produced in the A‐domain of HbpR, a protein belonging to the XylR family of σ(54)‐dependent transcription activators, with the purpose of changing its effector recognition specificity from 2‐hydroxybiphenyl (2‐HBP, the cognate effector) to 2‐chlorobiphenyl (2‐CBP). Mutations were introd...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Blackwell Publishing Ltd
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3864433/ https://ncbi.nlm.nih.gov/pubmed/21261823 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2007.00008.x |
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