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Mutant HbpR transcription activator isolation for 2‐chlorobiphenyl via green fluorescent protein‐based flow cytometry and cell sorting

Mutants were produced in the A‐domain of HbpR, a protein belonging to the XylR family of σ(54)‐dependent transcription activators, with the purpose of changing its effector recognition specificity from 2‐hydroxybiphenyl (2‐HBP, the cognate effector) to 2‐chlorobiphenyl (2‐CBP). Mutations were introd...

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Detalhes bibliográficos
Main Authors: Beggah, Siham, Vogne, Christelle, Zenaro, Elena, Van Der Meer, Jan Roelof
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Publishing Ltd 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3864433/
https://ncbi.nlm.nih.gov/pubmed/21261823
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2007.00008.x
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