Neutron-encoded Signatures Enable Product Ion Annotation From Tandem Mass Spectra

We report the use of neutron-encoded (NeuCode) stable isotope labeling of amino acids in cell culture for the purpose of C-terminal product ion annotation. Two NeuCode labeling isotopologues of lysine, (13)C(6)(15)N(2) and (2)H(8), which differ by 36 mDa, were metabolically embedded in a sample prot...

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Detalhes bibliográficos
Main Authors: Richards, Alicia L., Vincent, Catherine E., Guthals, Adrian, Rose, Christopher M., Westphall, Michael S., Bandeira, Nuno, Coon, Joshua J.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2013
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3861726/
https://ncbi.nlm.nih.gov/pubmed/24043425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M113.028951
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