The Acquisition and Analysis of Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Data

Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single molecules. polTIRFM determines the three-dimensional angular orientation and the extent of wobble of a fluorescent probe bound to the macromolecule o...

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Bibliographic Details
Main Authors: Beausang, John F., Sun, Yujie, Quinlan, Margot E., Forkey, Joseph N., Goldman, Yale E.
Format: Artigo
Language:Inglês
Published: 2012
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3855859/
https://ncbi.nlm.nih.gov/pubmed/22661430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot069419
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