Comparison of RNA- or LNA-hybrid oligonucleotides in template-switching reactions for high-speed sequencing library preparation

BACKGROUND: Analyzing the RNA pool or transcription start sites requires effective means to convert RNA into cDNA libraries for digital expression counting. With current high-speed sequencers, it is necessary to flank the cDNAs with specific adapters. Adding template-switching oligonucleotides to re...

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Библиографические подробности
Главные авторы: Harbers, Matthias, Kato, Sachi, de Hoon, Michiel, Hayashizaki, Yoshihide, Carninci, Piero, Plessy, Charles
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2013
Предметы:
Methodology Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3853366/
https://ncbi.nlm.nih.gov/pubmed/24079827
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-14-665
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