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Assessment of transcript reconstruction methods for RNA-seq

We evaluated 25 protocol variants of 14 independent computational methods for exon identification, transcript reconstruction and expression-level quantification from RNA-seq data. Our results show that most algorithms are able to identify discrete transcript components with high success rates but th...

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Détails bibliographiques
Auteurs principaux: Steijger, Tamara, Abril, Josep F, Engström, Pär G, Kokocinski, Felix, Hubbard, Tim J, Guigó, Roderic, Harrow, Jennifer, Bertone, Paul
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group US 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3851240/
https://ncbi.nlm.nih.gov/pubmed/24185837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2714
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