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Simultaneous splicing of multiple DNA fragments in one PCR reaction
BACKGROUND: Rapid and simultaneous splicing of multiple DNA fragments is frequently required in many recombinant DNA projects. However, former overlap extension PCRs, the most common methods for splicing DNA fragments, are not really simultaneous fusing of multiple DNA fragments. RESULTS: We perform...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3847634/ https://ncbi.nlm.nih.gov/pubmed/24015676 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1480-9222-15-9 |
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