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Robust 3D DNA FISH Using Directly Labeled Probes

3D DNA FISH has become a major tool for analyzing three-dimensional organization of the nucleus, and several variations of the technique have been published. In this article we describe a protocol which has been optimized for robustness, reproducibility, and ease of use. Brightly fluorescent directl...

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Détails bibliographiques
Auteurs principaux: Bolland, Daniel J., King, Michelle R., Reik, Wolf, Corcoran, Anne E., Krueger, Christel
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3846859/
https://ncbi.nlm.nih.gov/pubmed/23978815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50587
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