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A modified TALEN-based system for robust generation of knock-out human pluripotent stem cell lines and disease models

BACKGROUND: Transcription activator-like effector nucleases (TALENs) have emerged as a tool for enabling targeted gene editing and disruption in difficult systems, such as human pluripotent stem cells (hPSCs). The modular architecture of TAL effectors theoretically enables targeting of any genomic l...

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Détails bibliographiques
Auteurs principaux: Frank, Stefan, Skryabin, Boris V, Greber, Boris
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3840567/
https://ncbi.nlm.nih.gov/pubmed/24206569
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-14-773
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