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Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.

We have developed an Escherichia coli plasmid vector for the identification and expression of foreign DNA segments that are open reading frames (ORFs). The 5' end of ompF, an E. coli gene encoding an abundant outer membrane protein, is used to provide a strong, regulated promoter, translation i...

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Detalhes bibliográficos
Main Authors: Weinstock, G M, ap Rhys, C, Berman, M L, Hampar, B, Jackson, D, Silhavy, T J, Weisemann, J, Zweig, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC384052/
https://ncbi.nlm.nih.gov/pubmed/6308625
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