Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.

We have developed an Escherichia coli plasmid vector for the identification and expression of foreign DNA segments that are open reading frames (ORFs). The 5' end of ompF, an E. coli gene encoding an abundant outer membrane protein, is used to provide a strong, regulated promoter, translation i...

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Main Authors: Weinstock, G M, ap Rhys, C, Berman, M L, Hampar, B, Jackson, D, Silhavy, T J, Weisemann, J, Zweig, M
格式: Artigo
語言:Inglês
出版: 1983
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC384052/
https://ncbi.nlm.nih.gov/pubmed/6308625
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