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Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli.

We have obtained cDNA clones that contain the entire coding region of the human purine-nucleoside phosphorylase (PNP; EC 2.4.2.1) mRNA. The cDNA sequences were generated by reverse transcription of PNP-enriched mRNA obtained by immunoadsorption of HeLa cell polyribosomes with monospecific antibody t...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Goddard, J M, Caput, D, Williams, S R, Martin, D W
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC384021/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6410388/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.14.4281
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