Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9

The ability to engineer genomes in a specific, systematic, and cost-effective way is critical for functional genomic studies. Recent advances using the CRISPR-associated single-guide RNA system (Cas9/sgRNA) illustrate the potential of this simple system for genome engineering in a number of organism...

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Bibliographic Details
Main Authors: Ren, Xingjie, Sun, Jin, Housden, Benjamin E., Hu, Yanhui, Roesel, Charles, Lin, Shuailiang, Liu, Lu-Ping, Yang, Zhihao, Mao, Decai, Sun, Lingzhu, Wu, Qujie, Ji, Jun-Yuan, Xi, Jianzhong, Mohr, Stephanie E., Xu, Jiang, Perrimon, Norbert, Ni, Jian-Quan
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2013
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3839733/
https://ncbi.nlm.nih.gov/pubmed/24191015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1318481110
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