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High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene
Hybrid ColE1 plasmids, containing cloned DNA from the yeast ARG4 region [e.g., pYe(arg4)1], transform yeast arg4 mutants to ARG4(+) with a frequency of 10(-4) (about 10(3) transformants per μg of plasmid DNA) and can replicate autonomously without integrating into the yeast genome. The yeast transfo...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1979
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC383928/ https://ncbi.nlm.nih.gov/pubmed/386351 |
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