Transformation of Neurospora crassa with recombinant plasmids containing the cloned glutamate dehydrogenase (am) gene: evidence for autonomous replication of the transforming plasmid.

We have characterized Neurospora crassa transformants obtained with plasmid pJR2, which consists of the Neurospora glutamate dehydrogenase (am) gene cloned in pUC8 and an am132 host strain which contains a deletion encompassing the cloned fragment. Every one of 33 transformants tested showed extreme...

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Detalhes bibliográficos
Main Authors: Grant, D M, Lambowitz, A M, Rambosek, J A, Kinsey, J A
Formato: Artigo
Idioma:Inglês
Publicado em: 1984
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC369021/
https://ncbi.nlm.nih.gov/pubmed/6095037
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