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Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding
Cysteine is an extremely useful site for selective attachment of labels to proteins for many applications, including the study of protein structure in solution by electron paramagnetic resonance (EPR), fluorescence spectroscopy and medical imaging. The demand for quantitative data for these applicat...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3836627/ https://ncbi.nlm.nih.gov/pubmed/24091556 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.128 |
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