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Total chemical synthesis of human proinsulin()

A convergent synthetic strategy based on modern chemical ligation methods was used to make human proinsulin. The synthetic protein was characterized by LCMS, CD spectroscopy, and by 1D- and 2D-NMR spectroscopy. Synthetic human proinsulin had full biochemical activity in a receptor-binding assay.

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Autors principals: Luisier, Samuel, Avital-Shmilovici, Michal, Weiss, Michael A., Kent, Stephen B. H.
Format: Artigo
Idioma:Inglês
Publicat: 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3835593/
https://ncbi.nlm.nih.gov/pubmed/20877850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc03141k
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