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Total chemical synthesis of human proinsulin()
A convergent synthetic strategy based on modern chemical ligation methods was used to make human proinsulin. The synthetic protein was characterized by LCMS, CD spectroscopy, and by 1D- and 2D-NMR spectroscopy. Synthetic human proinsulin had full biochemical activity in a receptor-binding assay.
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3835593/ https://ncbi.nlm.nih.gov/pubmed/20877850 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc03141k |
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