A Quantitative Proteomic Workflow for Characterization of Frozen Clinical Biopsies: Laser Capture Microdissection Coupled with Label-Free Mass Spectrometry

This paper describes a simple, highly efficient and robust proteomic workflow for routine liquid-chromatography tandem mass spectrometry analysis of Laser Microdissection Pressure Catapulting (LMPC) isolates. Highly efficient protein recovery was achieved by optimization of a “one-pot” protein extra...

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Hauptverfasser: Shapiro, John P., Biswas, Sabyasachi, Merchant, Anand S., Satoskar, Anjali, Taslim, Cenny, Lin, Shili, Rovin, Brad H., Sen, Chandan K., Roy, Sashwati, Freitas, Michael A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3835202/
https://ncbi.nlm.nih.gov/pubmed/23022584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2012.09.019
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