Isolation of lysosomal α-mannosidase mutants of Chinese hamster ovary cells
A method is presented for detection of lysosomal hydrolase activity in situ in colonies of Chinese hamster ovary cells. This method was used to screen for mutants deficient in lysosomal α-mannosidase. Mutagenized cells were replicated onto filter papers [Esko, J. D. & Raetz, C. R. H. (1978) Proc. Na...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteur principal: | |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1979
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383502/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/287030/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1911 |
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