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Isolation of lysosomal α-mannosidase mutants of Chinese hamster ovary cells

A method is presented for detection of lysosomal hydrolase activity in situ in colonies of Chinese hamster ovary cells. This method was used to screen for mutants deficient in lysosomal α-mannosidase. Mutagenized cells were replicated onto filter papers [Esko, J. D. & Raetz, C. R. H. (1978) Proc...

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Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Glavni avtor: Robbins, April R.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1979
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383502/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/287030/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1911
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