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Directed evolution of the TALE N-terminal domain for recognition of all 5′ bases

Transcription activator-like effector (TALE) proteins can be designed to bind virtually any DNA sequence. General guidelines for design of TALE DNA-binding domains suggest that the 5′-most base of the DNA sequence bound by the TALE (the N(0) base) should be a thymine. We quantified the N(0) requirem...

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Detalles Bibliográficos
Main Authors: Lamb, Brian M., Mercer, Andrew C., Barbas, Carlos F.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3834825/
https://ncbi.nlm.nih.gov/pubmed/23980031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt754
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